Get All The Information About Galectin-3 ELISA Kit

The Galectin-3 ELISA Kit is Sandwich High Sensitivity and is used for the detection of Galectin-3/LGALS3 in human beings. There are 96 Wells available in one package, and that comes along with removable strips. This product has been cited in around five publications.

Quick Facts About Galectin-3 ELISA Kit

Some of the information about the Galectin-3 ELISA Kit is as follows:

  • Size: 96wells in a kit, with removable strips.
  • SKU: EK0764.
  • Sample Type: cell culture supernates, serum and plasma (heparin, EDTA), cell lysates.
  • Reactivity: Human
  • Sample Volume: 100μl per well
  • Assay Range: 156pg/ml-10000pg/ml
  • Sensitivity: <10pg/ml

Product Information of Galectin-3 ELISA Kit

These are some of the required facts about the Galectin-3 ELISA Kit:

  • Species of reactivity: Humans
  • Storage: It may be stored at 4°C for six months and -20°C for 12 months. The product must not be repeatedly frozen and thawed in cycles.
  • Immunogen: Immunogen sequence: A2-I250, expression system for standard: E.coli;.
  • Cross Reactivity: This protein does not show any significant cross-reactivity with other proteins.
  • Antibody Clonalities: Detection antibody

(monoclonal antibody from mouse), (polyclonal antibody from goat), capture antibody.

  • Application: This product can be applied for ELISA and used in both positive as well as negative control designs.

Product Description

This Detect Human Galectin 3/LGALS3 has a sensitivity level of less than 10pg/ml. It comes with 96 wells in a single kit along with a number of removable strips. It is a sandwich ELISA kit, which works fast in terms of assay time and is a TMB colorimetric sandwich kit. It is compatible with cell lysates, cell culture supernates, as well as the serum and plasma (heparin, EDTA). A significant expression of the Galectin-3 specificity is located in the colonic epithelium. This protein is also abundantly found in macrophages as well as the fetal membranes at times.

Preliminary Information about LGALS3

Some of the most basic information about the Galectin-3/LGALS3 are as follows:

  • Uniprot Id: P17931
  • Gene Name: LGALS3
  • NCBI Gene Id: 3958
  • Protein Name: Galectin-3
  • Molecular Weight: 26152
  • Alternative names: Galectin-3, Carbohydrate-binding protein 35, GALIG,

Laminin-binding protein; lectin, galactoside-binding, soluble, 3; Mac-2 antigen, CBP 35,35 kDa lectin, and more.

  • Post Translational Modifications: oxidation, methylation, demethylation, phosphorylation, glycosylation, cleavage.
  • Gene Location: 14q22.3, on Chromosome 14, gene sequence: NC_000014.9 (55129252..55145430)
  • Subcellular Localization: nucleus, cytoplasm, Secreted by non-classical secretory pathway, found in the surface of a cell.

Protein Functions

Galactose-specific lectin is known to bind with IgE. It is also known to mediate with the alpha-3, beta-1 integrin, along with the stimulation of CSPG4 of the migration of the endothelial cells. It acts as a pre-mRNA splicing factor inside the nucleus. This protein, in association with DMBT1, is required for the terminal differentiation of columnar epithelial cells during the phase of embryogenesis. This protein also plays a role in producing acute inflammatory responses. It helps in neutrophil activation and adhesion, opsonization of apoptotic neutrophils, chemoattraction of monocytes and macrophages as well as activation of mast cells. Some of its interacting proteins are ALCAM, PRR13, SS18L1, MMP7, and more.